Characterization of 10S RNA: A new stable RNA molecule from Escherichia coli

  title={Characterization of 10S RNA: A new stable RNA molecule from Escherichia coli},
  author={Bimal K. Ray and David Apirion},
  journal={Molecular and General Genetics MGG},
  • B. Ray, D. Apirion
  • Published 2 July 1979
  • Biology
  • Molecular and General Genetics MGG
SummaryWhen cells of Escherichia coli are labeled with 32Pi for long periods of time and the cell content is subjected to electrophoresis in polyacrylamide gels, an RNA band appears which is about 10S in size. This band seems to contain three conformers. After treatment with formamide only a single band appears in this region of the gel, which contains 550 nucleotides as determined from its mobility. The complexity of the fingerprint of this material, after digestion with T1-RNase, is in… 
A precursor for a small stable RNA (10Sa RNA) ofEscherichia coli
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated, and it is shown that the larger RNA is converted to the smaller, mature RNA.
Structure and functional properties of prokaryotic small noncoding RNAs
This review summarizes recent data on the functions of prokaryotic sRNAs and approaches to their identification.
Contribution of the Second OB Fold of Ribosomal Protein S1 from Escherichia coli to the Recognition of TmRNA
The results suggest that the second OB fold in protein S1 is essential for the recognition of tmRNA, while the four C-terminal OB folds play a role in stabilizing the S1–tmRNA complex.
An overview of the bacterial SsrA system modulating intracellular protein levels and activities
The history, structural characteristics, and functional mechanisms of the SsrA tagging and degrading machineries, as well as their technical uses and limitations, are summarized.
Gene expression in Chromobacterium violaceum.
Analysis of the C. violaceum genome showed that transcription initiation, elongation and termination are performed by the five well-known RNA polymerase subunits, five categories of sigma 70 factors, one sigma 54 factor, as well as six auxiliary elongationand termination factors.
RNase P RNA ofMycoplasma capricolum
There are at least six small stable RNAs in Mycoplasma capricolum cells besides tRNAs and rRNAs, and one of them, MCS5 RNA, is a homolog of RNase P RNA, which is consistent with previous conclusions that mycoplasmas are phylogenetically related to the low G+C Gram-positive bacterial group.
Turnover of Endogenous SsrA-tagged Proteins Mediated by ATP-dependent Proteases in Escherichia coli*
It is concluded that ClpXP, together with SspB, is uniquely adapted for degradation of SsrA-tagged proteins and is responsible for the major part of their degradation in vivo.
tmRNA to the rescue: Structural motives for the salvage of stalled ribosomes
How structural studies have yielded a model for how the pre-translocation stages of trans-translation employingStructural mimicry employing structural mimicry may be carried out is recalled.
System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing
A protocol using high throughput sequencing to measure steady-state RNA abundances, RNA polymerase elongation rates and RNA degradation rates simultaneously with high nucleotide-resolution genome-wide is developed and shows that RNA has similar lifetime at all positions throughout the length of the transcript.


Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis.
Two-dimensional polyacrylamide gel electrophoresis was used to purify RNA in the 4 S to 5 S region and shows that during 20 min labeling the newly characterized molecules accumulate at levels comparable to many individual tRNAs.
Small stable RNA of Neurospora crassa
It seems that the N. crassa 7S rRNA is the largest low molecular weight ribosomal RNA studied thus far, as it migrates slower on polyacrylamide gels than the 6S RNA of E. coli, which contains 184 nucleotides.
Nucleotide Sequence of 5S-ribosomal RNA from Escherichia coli
Using a two-dimensional fractionation procedure for the separation of nucleotides labelled with phosphorus-32, the sequences of all the oligonucleotides obtained by complete digestion of 32P-labelled 5S RNA are determined and the unique sequence shown in Figs.
Small Stable RNAs from Escherichia coli: Evidence for the Existence of New Molecules and for a New Ribonucleoprotein Particle Containing 6S RNA
Small stable RNA molecules of Escherichia coli other than 5S (rRNA) and 4S (tRNA) were studied and 6S RNA was unique in that it seemed to exist in a ribonucleoprotein particle.
Studies and sequences of Escherichia coli 4.5 S RNA.
  • B. Griffin
  • Biology
    The Journal of biological chemistry
  • 1975