Characterization by flow cytometry of fluorescent, selective agonist probes of the A(3) adenosine receptor.

@article{Kozma2013CharacterizationBF,
  title={Characterization by flow cytometry of fluorescent, selective agonist probes of the A(3) adenosine receptor.},
  author={Eszter Kozma and Elizabeth T. Gizewski and Dilip K. Tosh and Lucia Squarcialupi and John A. Auchampach and Kenneth A. Jacobson},
  journal={Biochemical pharmacology},
  year={2013},
  volume={85 8},
  pages={1171-81}
}
Various fluorescent nucleoside agonists of the A3 adenosine receptor (AR) were compared as high affinity probes using radioligands and flow cytometry (FCM). They contained a fluorophore linked through the C2 or N(6) position and rigid A3AR-enhancing (N)-methanocarba modification. A hydrophobic C2-(1-pyrenyl) derivative MRS5704 bound nonselectively. C2-Tethered cyanine5-dye labeled MRS5218 bound selectively to hA3AR expressed in whole CHO cells and membranes. By FCM, binding was A3AR-mediated… CONTINUE READING

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