Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b

@article{Nishiguchi2004CharacterizationAS,
  title={Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b},
  author={Reiko Nishiguchi and Mituru Takanami and Atsuhiro Oka},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={206},
  pages={1-8}
}
Starting from a cosmid library of the 250 kb hairy root inducing plasmid pRiA 4b, a mini-pRiA 4b replicon of 4.6 kb was isolated. This mini-plasmid was stably maintained in Agrobacterium species and its replication characteristics, such as temperature-sensitive replication, copy number and incompatibility, were similar to those of the parental pRiA 4b. Analysis of deletion mutants indicated that almost the entire 4.6 kb region was required for autonomous replication. The nucleotide sequence of… CONTINUE READING
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