Characterization and regulation of beta 1-adrenergic receptors in a human neuroepithelioma cell line.

@article{Fishman1991CharacterizationAR,
  title={Characterization and regulation of beta 1-adrenergic receptors in a human neuroepithelioma cell line.},
  author={Peter Fishman and Eliezer Zachary Nussbaum and Ronald S Duman},
  journal={Journal of neurochemistry},
  year={1991},
  volume={56 2},
  pages={596-602}
}
Intact human neuroepithelioma SK-N-MC cells bound the beta-adrenergic antagonist (-)-[3H]-CGP 12177 with a KD of 0.13 nM and a Bmax of 17,500 sites/cell. When the cells were exposed to beta-adrenergic agonists, they accumulated cyclic AMP in the following order of potency: isoproterenol much greater than norepinephrine greater than epinephrine, which is indicative of a beta 1-subtype receptor. Membranes prepared from the cells bound (-)-3-[125I]iodocyanopindolol with a KD of 11.5 pM. Inhibition… CONTINUE READING