Characterization and quantification of diadenosine hexaphosphate in chromaffin cells: granular storage and secretagogue-induced release.

@article{Pintor1992CharacterizationAQ,
  title={Characterization and quantification of diadenosine hexaphosphate in chromaffin cells: granular storage and secretagogue-induced release.},
  author={Jes{\'u}s Pintor and Pedro Rotll{\'a}n and Manuel Torres and M Teresa Miras-Portugal},
  journal={Analytical biochemistry},
  year={1992},
  volume={200 2},
  pages={296-300}
}
The presence of diadenosine hexaphosphate (Ap6A) in chromaffin cells is described. The characterization of Ap6A has been accomplished by HPLC techniques, using three different elution conditions, rechromatography, and coelution with standards. Treatment with phosphodiesterase from Crotalus durissus produced AMP and adenosine pentaphosphate. The HPLC techniques described allowed the quantification of Ap6A in the picomole range. Chromaffin granules store Ap6A in a quantity of 48.5 +/- 9.7 nmol/mg… CONTINUE READING

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