Characterization and partial purification of squalene-2,3-oxide cyclase from Saccharomyces cerevisiae.

@article{Balliano1992CharacterizationAP,
  title={Characterization and partial purification of squalene-2,3-oxide cyclase from Saccharomyces cerevisiae.},
  author={Gianni Balliano and Franca Viola and Maurizio Ceruti and Luigi Cattel},
  journal={Archives of biochemistry and biophysics},
  year={1992},
  volume={293 1},
  pages={
          122-9
        }
}
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34 Citations
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6
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34 Citations

Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver.
The monotopic membrane protein human oxidosqualene cyclase is active as monomer.
Structure and function of a squalene cyclase.
TLDR
The crystal structure of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was determined and reveals a membrane protein with membrane-binding characteristics similar to those of prostaglandin-H2 synthase, the only other reported protein of this type.
The structure of the membrane protein squalene-hopene cyclase at 2.0 A resolution.
TLDR
The structure of the enzyme from Alicyclobacillus acidocaldarius has been determined and the membrane-binding interactions of which are described and compared with those of two prostaglandin-H2 synthase isoenzymes, the only other structurally characterized proteins of this type.
Purification and properties of the squalene-hopene cyclase from Rhodopseudomonas palustris, a purple non-sulfur bacterium producing hopanoids and tetrahymanol.
Synthesis and biological activity of a squalenoid maleimide and other classes of squalene derivatives as irreversible inhibitors of 2,3-oxidosqualene cyclase
Solubilization and identification of essential functional groups of Candida albicans oxidosqualene cyclase.
TLDR
It is shown that the C. albicans enzyme is much more heat-labile compared with Saccharomyces cerevisiae and rat liver cyclases, requires a histidyl residue for enzyme activity, contains an essential thiol residue either close to or in the active site and exhibits a carbocationic mechanism for catalysis.
Thiol-modifying inhibitors for understanding squalene cyclase function.
The function of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was studied by labelling critical cysteine residues of the enzyme, either native or inserted by site-directed mutagenesis,…
Yeast Oxidosqualene Cyclase (Erg7p) Is a Major Component of Lipid Particles*
TLDR
Evidence is provided that lipid particles are not only a depot for neutral lipids, but also participate in coordinate sterol metabolism and trafficking and serve as a storage site for compounds that may negatively affect membrane integrity.
Synthesis and biological activity of new lodoacetamide derivatives on mutants of squalene-hopene cyclase
TLDR
New iodoacetamide derivatives, containing a dodecyl or a squalenyl moiety, were synthesized and showed little activity toward either mutant, whereas N-squalenyliodoacetamide showed a stronger effect on the sextuple than on the quintuple mutant, as expected.
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References

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Characterization and partial purification of squalene-hopene cyclase from Bacillus acidocaldarius
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Comparative properties of 2,3-oxidosqualene-lanosterol cyclase from yeast and liver.
Partial purification of 2,3-oxidosqualene-lanosterol cyclase from hog-liver. Evidence for a functional thiol residue.
Purification and some properties of squalene-2,3-epoxide: lanosterol cyclase from rat liver.
Squalene-2,3-epoxide: lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The purified enzyme showed a single band on SDS-polyacrylamide gel…
Inhibition of sterol biosynthesis in Saccharomyces cerevisiae by N,N-diethylazasqualene and derivatives.
Isolation of phospholipase A2 from sheep erythrocyte membranes in the presence of detergents
Studies on the Δ5-Desaturation in Ergosterol Biosynthesis in Yeast
TLDR
Results suggested an involvement in delta 5-desaturation of a mixed function oxidase system resembling that for the fatty acyl-CoA desaturation reaction.
In vitro inhibition of animal and higher plants 2,3-oxidosqualene-sterol cyclases by 2-aza-2,3-dihydrosqualene and derivatives, and by other ammonium-containing molecules.
Membrane-surfactant interactions. The effect of Triton X-100 on sarcoplasmic reticulum vesicles.
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