Characterization and partial purification of squalene-2,3-oxide cyclase from Saccharomyces cerevisiae.

  title={Characterization and partial purification of squalene-2,3-oxide cyclase from Saccharomyces cerevisiae.},
  author={Gianni Balliano and Franca Viola and Maurizio Ceruti and Luigi Cattel},
  journal={Archives of biochemistry and biophysics},
  volume={293 1},
The monotopic membrane protein human oxidosqualene cyclase is active as monomer.
Structure and function of a squalene cyclase.
The crystal structure of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was determined and reveals a membrane protein with membrane-binding characteristics similar to those of prostaglandin-H2 synthase, the only other reported protein of this type.
The structure of the membrane protein squalene-hopene cyclase at 2.0 A resolution.
The structure of the enzyme from Alicyclobacillus acidocaldarius has been determined and the membrane-binding interactions of which are described and compared with those of two prostaglandin-H2 synthase isoenzymes, the only other structurally characterized proteins of this type.
Solubilization and identification of essential functional groups of Candida albicans oxidosqualene cyclase.
It is shown that the C. albicans enzyme is much more heat-labile compared with Saccharomyces cerevisiae and rat liver cyclases, requires a histidyl residue for enzyme activity, contains an essential thiol residue either close to or in the active site and exhibits a carbocationic mechanism for catalysis.
Thiol-modifying inhibitors for understanding squalene cyclase function.
The function of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was studied by labelling critical cysteine residues of the enzyme, either native or inserted by site-directed mutagenesis,
Yeast Oxidosqualene Cyclase (Erg7p) Is a Major Component of Lipid Particles*
Evidence is provided that lipid particles are not only a depot for neutral lipids, but also participate in coordinate sterol metabolism and trafficking and serve as a storage site for compounds that may negatively affect membrane integrity.
Synthesis and biological activity of new lodoacetamide derivatives on mutants of squalene-hopene cyclase
New iodoacetamide derivatives, containing a dodecyl or a squalenyl moiety, were synthesized and showed little activity toward either mutant, whereas N-squalenyliodoacetamide showed a stronger effect on the sextuple than on the quintuple mutant, as expected.


Purification and some properties of squalene-2,3-epoxide: lanosterol cyclase from rat liver.
Squalene-2,3-epoxide: lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The purified enzyme showed a single band on SDS-polyacrylamide gel
Studies on the Δ5-Desaturation in Ergosterol Biosynthesis in Yeast
Results suggested an involvement in delta 5-desaturation of a mixed function oxidase system resembling that for the fatty acyl-CoA desaturation reaction.