Characterization and multi-generational stability of the growth hormone transgene (EO-1α) responsible for enhanced growth rates in Atlantic Salmon

  title={Characterization and multi-generational stability of the growth hormone transgene (EO-1$\alpha$) responsible for enhanced growth rates in Atlantic Salmon},
  author={Edward S. Yaskowiak and Margaret A. Shears and Alka Agarwal-Mawal and Garth L. Fletcher},
  journal={Transgenic Research},
Transgenic technologies provide a promising means by which desirable traits can be introduced into cultured fish species within a single generation thus accelerating the production of genetically superior broodstock for aquaculture. However, before such fish are allowed to be marketed as food they must receive government regulatory approval. Two pivotal regulatory requirements are: (1) complete characterization of the genomically integrated transgene and, (2) demonstration that the transgene… 

Tissue specific expression of antifreeze protein and growth hormone transgenes driven by the ocean pout (Macrozoarces americanus) antifreeze protein OP5a gene promoter in Atlantic salmon (Salmo salar)

It is demonstrated that the AFP and GH trangenes were expressed in almost all body tissues, suggesting that the promoter region of the OP5a AFP gene lacks tissue specific elements.

Autotransgenic and allotransgenic manipulation of growth traits in fish for aquaculture: a review

This review considers the generation, development and prospects of autotransgenic manipulation of fish growth, comparing the growth performance of currently available autOTransgenic fish strains with those of relevant fast-growing allotransgenic fishes.

The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

Genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp.

Delayed Phenotypic Expression of Growth Hormone Transgenesis during Early Ontogeny in Atlantic Salmon (Salmo salar)?

It is suggested that biologically significant differences in fitness-related traits between GH transgenic and non-transgenic Atlantic salmon were less than family differences during the earliest life stages, and that family differences were more important in explaining phenotypic variation.

[Progress in transgenic fish techniques and application].

The progress in transgenic fish research and ecological assessments is summarized and it is critical to establish highly naturalized environments for acquiring reliable data that can be used to evaluate the environ-mental risk.

Lysozyme transgenic Atlantic salmon (Salmo salar L.)

A line of transgenic Atlantic salmon is generated using a gene construct consisting of a rainbow trout lysozyme gene under the control of the ocean pout antifreeze protein gene promoter op5a (opAFP-rtLys), and the transgene exhibited Mendelian inheritance at both the F1 and the F2 generations and demonstrated that it had integrated into a single chromosome in the genetic founder.

Reproductive performance of alternative male phenotypes of growth hormone transgenic Atlantic salmon (Salmo salar)

Although transgenic males displayed reduced breeding performance relative to nontransgenics, both male reproductive phenotypes demonstrated the ability to participate in natural spawning events and thus have the potential to contribute genes to subsequent generations.



Transgenic salmon: potential and hurdles.

This brief communication centres around personal experiences with Atlantic salmon and outlines the plans and progress towards demonstrating the safety of transgenic fish to the consumer and to the environment.

Gene transfer: potential to enhance the genome of Atlantic salmon for aquaculture

The experience with the regulatory authorities, the industry and the press indicates that the successful introduction of transgenic salmon into the aquaculture industry involves issues concerning not only science but also food safety, environmental safety, animal welfare and consumer acceptance.

Fish can be First – Advances in Fish Transgenesis for Commercial Applications

  • H. Żbikowska
  • Biology, Environmental Science
    Transgenic Research
  • 2004
‘All-fish’ growth hormone gene constructs and antifreeze protein genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate and an increase in cold and freeze tolerance.

Growth enhancement in transgenic tilapia by ectopic expression of tilapia growth hormone.

Results showed that low-level ectopic expression of tiGH resulted in a growth acceleration in transgenic tilapia, indicating that Tilapia GH gene transfer is an alternative for growth Acceleration intilapia.

Growth Enhancement in Transgenic Atlantic Salmon by the Use of an “All Fish” Chimeric Growth Hormone Gene Construct

An “all fish” growth hormone (GH) chimeric gene construct is developed by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone to generate transgenic Atlantic salmon.

Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene

A digestibility trial suggested that transgenic tilapia were more efficient utilizers of protein, dry matter and energy and apparent digestibility of protein and apparent energy digestibility were higher in the transgenic fish.

Functional analysis and temporal expression of promoter regions from fish antifreeze protein genes in transgenic Japanese medaka embryos.

These studies demonstrated that the AFP genes have functional promoter regions containing positive as well as negative regulatory regions, indicating that these genes could be regulated at multiple sites and observed a promoter-specific pattern of temporal expression.

Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

It is shown that each F4 transgenic fish contained about 200 copies of the pMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites.

Embryonic and genetic manipulation in fish

Combination of techniques of fish cell culture, gene transformation with cultured cells and nuclear transplantation should be able to generate homogeneous strain of valuable transgenic fish to fulfil human requirement in 21st century.