Characterization and localization of metal-responsive-element-binding transcription factors from tilapia.

@article{Cheung2010CharacterizationAL,
  title={Characterization and localization of metal-responsive-element-binding transcription factors from tilapia.},
  author={Andrew Pok-Lap Cheung and Candy Yee-Man Au and William Chan and King Ming Chan},
  journal={Aquatic toxicology},
  year={2010},
  volume={99 1},
  pages={42-55}
}
Two isoforms of MTF-1, MTF-1L (long form) and MTF-1S (short form), were cloned in tilapia (Ti) and characterized in a tilapia liver cell line, Hepa-T1. The cloned tiMTF-1L has the characteristics of all of the tiMTF-1S identified so far with the zinc finger domain having six fingers, the acidic-rich, proline-rich, and serine/threonine-rich domains; however, the short form encodes for the zinc finger domain with five zinc fingers only and no other domains. The transient transfection of tiMTF-1L… CONTINUE READING