Characterization and localization of cannabinoid receptors in rat brain: a quantitative in vitro autoradiographic study

@inproceedings{Herkenham1991CharacterizationAL,
  title={Characterization and localization of cannabinoid receptors in rat brain: a quantitative in vitro autoradiographic study},
  author={Miles Herkenham and Abigail K. Lynn and M.Ross Johnson and LS Melvin and B de Costa and KC Rice},
  booktitle={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1991}
}
A potent, synthetic cannabinoid was radiolabeled and used to characterize and precisely localize cannabinoid receptors in slide- mounted sections of rat brain and pituitary. Assay conditions for 3H- CP55,940 binding in Tris-HCl buffer with 5% BSA were optimized, association and dissociation rate constants determined, and the equilibrium dissociation constant (Kd) calculated (21 nM by liquid scintillation counting, 5.2 nM by quantitative autoradiography). The results of competition studies… Expand
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  • Proceedings of the National Academy of Sciences of the United States of America
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The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in the in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience. Expand
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