Characterization and localization of cannabinoid receptors in rat brain: a quantitative in vitro autoradiographic study.

@article{Herkenham1991CharacterizationAL,
  title={Characterization and localization of cannabinoid receptors in rat brain: a quantitative in vitro autoradiographic study.},
  author={Miles Herkenham and A B Lynn and Martin R. Johnson and Lawrence S. Melvin and B R de Costa and Kenner C. Rice},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={1991},
  volume={11 2},
  pages={563-83}
}
A potent, synthetic cannabinoid was radiolabeled and used to characterize and precisely localize cannabinoid receptors in slide-mounted sections of rat brain and pituitary. Assay conditions for 3H-CP55,940 binding in Tris-HCl buffer with 5% BSA were optimized, association and dissociation rate constants determined, and the equilibrium dissociation constant (Kd) calculated (21 nM by liquid scintillation counting, 5.2 nM by quantitative autoradiography). The results of competition studies, using… CONTINUE READING
Highly Influential
This paper has highly influenced 90 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
681 Citations
99 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 681 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 99 references

The discovery of nonclassical cannabinoid analgetics

  • MR Johnson, LS Melvin
  • Cannabinoids as therapeutic agents ( Mech - oulam…
  • 1986
Highly Influential
15 Excerpts

Cannabis. Its chemistry, pharmacology, and toxicology

  • LS Harris, WL Dewey, RK Razdan
  • Handbook of experimental pharmacology (
  • 1977
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…