Characterization and inhibitor sensitivity of human sperm phospholipase A2: evidence against pivotal involvement of phospholipase A2 in the acrosome reaction.

@article{Anderson1990CharacterizationAI,
  title={Characterization and inhibitor sensitivity of human sperm phospholipase A2: evidence against pivotal involvement of phospholipase A2 in the acrosome reaction.},
  author={Robert A. Anderson and Sarah K Johnson and P Bielfeld and Kenneth A. Feathergill and Lourens J. D. Zaneveld},
  journal={Molecular reproduction and development},
  year={1990},
  volume={27 4},
  pages={305-25}
}
The kinetic properties and inhibitor sensitivity of human sperm phospholipase A2 (PLA2; EC 3.1.1.4) were studied. Phospholipase activity was isolated from human spermatozoa by acid extraction. Hydrolysis of dipalmitoyl phosphatidylcholine was specific to the sn-2 position. Activity was sensitive to product inhibition (60% inhibition by 0.1 mM lysophosphatidylcholine). The effects of Ca2+ and sodium deoxycholate on enzyme activity were biphasic; maximal activities were observed at 0.5 mM… CONTINUE READING