Characterization and immunogenicity of a candidate subunit vaccine against varicella-zoster virus

Abstract

This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specific glycoproteins, while virus DNA was less than 20 pg/250 μg protein — a putative vaccine dose. The vaccine was immunogenic in rabbits and stimulated antibodies to the major capsid protein as well as to glycoproteins; an immunoprecipitin was shared with a known immune human serum. The preparation contained no infectious VZV with no evidence of side effects in a rabbit or in five human vaccinees during a follow-up period of 6–10 years.

DOI: 10.1007/BF00277161

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Cite this paper

@article{Davies1994CharacterizationAI, title={Characterization and immunogenicity of a candidate subunit vaccine against varicella-zoster virus}, author={Jonathan I. Davies and J. A. Hallworth and Phillippa McLeish and Stephen Randall and Brian Martin and Alastair Buchan and Gill Skinner}, journal={Medical Microbiology and Immunology}, year={1994}, volume={183}, pages={105-117} }