Characterization and immunocytochemical application of monoclonal antibodies against enkephalins.

  title={Characterization and immunocytochemical application of monoclonal antibodies against enkephalins.},
  author={Augusto Claudio Cuello and C{\'e}sar Milstein and R{\'e}jean Couture and Barbara D. Wright and John V. Priestley and James Jarvis},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  volume={32 9},
Monoclonal antibodies were produced following immunization of mice with either [Leu5]enkephalin-bovine serum albumin or [Met5]enkephalin-keyhold limpet hemocyanin conjugates. Two monoclonal antibodies coded NOC1 and NOC2, respectively, were derived. These monoclonal antibodies did not discriminate between Leu- and Met-enkephalin in either radioimmunoassay or immunocytochemistry. NOC1 was characterized in detail. In radioimmunoassay NOC1 displayed about 40% crossreactivity with C-terminal… CONTINUE READING


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