Characterization and immobilization of trypsin on tannic acid modified Fe3O4 nanoparticles.

@article{Atacan2015CharacterizationAI,
  title={Characterization and immobilization of trypsin on tannic acid modified Fe3O4 nanoparticles.},
  author={Keziban Atacan and Mahmut {\"O}zacar},
  journal={Colloids and surfaces. B, Biointerfaces},
  year={2015},
  volume={128},
  pages={227-36}
}
Fe3O4 nanoparticles (NPs) were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. Fe3O4 NPs functionalized with tannic acid were prepared. After functionalization process, trypsin enzyme was immobilized on these Fe3O4 NPs. The influence of pH, temperature, thermal stability, storage time stability and reusability on non-covalent immobilization was studied. The properties of Fe3O4 and its modified forms were examined by scanning electron microscopy (SEM), Fourier transform… CONTINUE READING