Characterization and imaging of A6 epithelial cell clones expressing fluorescently labeled ENaC subunits.

Abstract

A6 model renal epithelial cells were stably transfected with enhanced green fluorescent protein (EGFP)-tagged alpha- or beta-subunits of the epithelial Na(+) channel (ENaC). Transfected RNA and proteins were both expressed in low abundance, similar to the endogenous levels of ENaC in native cells. In living cells, laser scanning confocal microscopy revealed… (More)

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@article{BlazerYost2001CharacterizationAI, title={Characterization and imaging of A6 epithelial cell clones expressing fluorescently labeled ENaC subunits.}, author={Bonnie L. Blazer-Yost and Michael Butterworth and Angela D Hartman and Gretchen E. Parker and Carla J Faletti and Willem J. Els and Simon Rhodes}, journal={American journal of physiology. Cell physiology}, year={2001}, volume={281 2}, pages={C624-32} }