Characterization and identification of a human dentin phosphophoryn

  title={Characterization and identification of a human dentin phosphophoryn},
  author={Sarah R. Chang and Dr. Daniel J. Chiego and Brian H Clarkson},
  journal={Calcified Tissue International},
The present study further characterizes an extract from immature, human tooth apicies from which an intact dentin phosphoprotein has been identified. Third molar apicies from developing roots were decalcified in 10% EDTA until Ca2+ was undetectable in the decalcifying solution. The crude extract was run on 7.5% SDS-PAGE and stained with “Stains-All.” Four distinct bands were found and the molecular weights were 140,60, 50, and 34 k. When run on a SDS-PAGE under nonreducing conditions the 60, 50… CONTINUE READING


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