Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei.

@article{Chen2015CharacterizationAH,
  title={Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei.},
  author={Fei Chen and Xiuzhen Chen and L Qin and Yong Tao and Zhi-yang Dong},
  journal={Biochemical and biophysical research communications},
  year={2015},
  volume={459 2},
  pages={
          184-188
        }
}
Trichoderma reesei is thought to be a promising recombinant host for the production and secretion of complex proteins due to its ability to secrete large amounts of proteins. In this study we identified a functional N-acetyl-β-glucosaminidase (NAGase) gene Nag1 in T. reesei. Nag1, a putative gene encoding a GH 20 family NAGase in T. reesei, was cloned and homologous overexpressed in the T. reesei RutC30ΔU3 with a strong cellobiohydrolase1 gene (cbh1) promoter. Nag1 was secreted in its active… CONTINUE READING

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