Characterization and functional complementation of a nonlethal deletion in the chromosome of a beta-glycosidase mutant of Sulfolobus solfataricus.

@article{Bartolucci2003CharacterizationAF,
  title={Characterization and functional complementation of a nonlethal deletion in the chromosome of a beta-glycosidase mutant of Sulfolobus solfataricus.},
  author={Simonetta Bartolucci and Mos{\'e} Rossi and Raffaele Cannio},
  journal={Journal of bacteriology},
  year={2003},
  volume={185 13},
  pages={3948-57}
}
LacS(-) mutants of Sulfolobus solfataricus defective in beta-glycosidase activity were isolated in order to explore genomic instability and exploit novel strategies for transformation and complementation. One of the mutants showed a stable phenotype with no reversion; analysis of its chromosome revealed the total absence of the beta-glycosidase gene (lacS). Fine mapping performed in comparison to the genomic sequence of S. solfataricus P2 indicated an extended deletion of approximately 13 kb… CONTINUE READING
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