Characterization and formation of the glutathione conjugate of clofibric acid.

@article{Shore1995CharacterizationAF,
  title={Characterization and formation of the glutathione conjugate of clofibric acid.},
  author={L J Shore and Catherine Fenselau and Angus R King and Ronald G. Dickinson},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1995},
  volume={23 1},
  pages={119-23}
}
The incubation of 1-O-clofibryl glucuronide (1-O-CAG), a metabolite of clofibrate, with glutathione (GSH) resulted in the appearance of a new peak when analyzed by HPLC. The use of HPLC coupled to electrospray-MS permitted the identification of the peak as S-(p-chlorophenoxy-2-methylpropanoyl)glutathione (CA-SG), formed by nucleophilic displacement of the glucuronide moiety from 1-O-CAG. Conjugate formation was enhanced 8-fold by rat liver glutathione S-transferases (GSTs). GSH was unreactive… CONTINUE READING