Characterization and distribution of extracellular matrix components and receptors in GH3B6 prolactin cells.

@article{Carvalho2000CharacterizationAD,
  title={Characterization and distribution of extracellular matrix components and receptors in GH3B6 prolactin cells.},
  author={D F de Carvalho and Karina Lani Silva and D A de Oliveria and D{\'e}a Maria Serra Villa-Verde and Henrique S{\'e}rgio Moraes Coelho and Luiz Cl{\'a}udio F Silva and Luiz E. Nasciutti},
  journal={Biology of the cell},
  year={2000},
  volume={92 5},
  pages={351-62}
}
GH3B6 cells, a rat pituitary tumor cell line, synthesize and secrete large amounts of prolactin (PRL) in vitro. In the present work, we evaluated the capacity of these cells to express extracellular matrix (ECM) components and receptors in vitro. The expression of laminin (LN), fibronectin (FN) and type IV collagen (CIV) was investigated by immunofluorescence assays. In comparison to PRL distribution, where around 50-70% of the cells contained PRL concentrated in the Golgi region, a variable… CONTINUE READING