Characterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.

  title={Characterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.},
  author={D. Roberts and G. Wright and L. Affronti and M. Reich},
  journal={Infection and immunity},
  volume={6 4},
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four strains), M. bovis strain BCG, M. scrofulaceum, and M. phlei, were assayed by 2D-IEP with four anti… Expand
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