Characterization and comparison of intercellular adherent junctions expressed by human corneal endothelial cells in vivo and in vitro.

@article{Zhu2008CharacterizationAC,
  title={Characterization and comparison of intercellular adherent junctions expressed by human corneal endothelial cells in vivo and in vitro.},
  author={Yingting Zhu and Yasutaka Hayashida and Ahmad Kheirkhah and Hua He and Szu-yu Chen and Scheffer C. G. Tseng},
  journal={Investigative ophthalmology & visual science},
  year={2008},
  volume={49 9},
  pages={3879-86}
}
PURPOSE Human corneal endothelial cell (HCEC) proliferation is controlled by HCEC junctions, but the mechanism of proliferation remains unknown. The authors sought to characterize adherent junction components of in vivo HCECs and to compare their gene expression and their proliferative potential with those of in vitro counterparts. METHODS Stripped human Descemet membranes were digested with collagenase A, and the resultant HCEC aggregates were cultured for 7, 14, and 21 days in supplemented… CONTINUE READING