Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.

@article{Cavaloc1994CharacterizationAC,
  title={Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.},
  author={Yvon Cavaloc and Michel Popielarz and Jean-Paul Fuchs and Renata Gattoni and James St{\'e}venin},
  journal={The EMBO Journal},
  year={1994},
  volume={13}
}
By adopting a monoclonal antibody approach, we have identified a novel splicing factor of 35 kDa which we have termed 9G8. The isolation and characterization of cDNA clones indicate that 9G8 is a novel member of the serine/arginine (SR) splicing factor family because it includes an N‐terminal RNA binding domain (RBD) and a C‐terminal SR domain. The RNA binding domain of 9G8 is highly homologous to those of the SRp20 and RBP1 factors (79‐71% identity), but the homology is less pronounced in the… 
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156 Citations

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TLDR
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Characterization of SRp46, a Novel Human SR Splicing Factor Encoded by a PR264/SC35 Retropseudogene
TLDR
It is demonstrated, for the first time, that an SR splicing factor, which represents a novel member of the SR family, is encoded by a functional retropseudogene.
A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities
TLDR
Results show that atRSZp22 is a true plant splicing factor which combines structural and functional features of both h9G8 and hSRp20 and RNA binding assays to selected RNA sequences indicate an RNA binding specificity similar to the human splicing factors 9g8 and SRp20.
Identification and Characterization of a Novel Serine-Arginine-Rich Splicing Regulatory Protein
ABSTRACT We have identified an 86-kDa protein containing a single amino-terminal RNA recognition motif and two carboxy-terminal domains rich in serine-arginine (SR) dipeptides. Despite structural…
Cloning of a gene (SR-A1), encoding for a new member of the human Ser/Arg-rich family of pre-mRNA splicing factors: overexpression in aggressive ovarian cancer
TLDR
The data suggest that this gene is overexpressed in a subset of ovarian cancers which are clinically more aggressive, and the mRNA of the SR-A1 gene in these cell lines appears to increase by estrogens, androgens and glucocorticoids, and to a lesser extend by progestins.
A Serine/Arginine-rich Domain in the Human U1 70k Protein Is Necessary and Sufficient for ASF/SF2 Binding*
TLDR
The segment in the human U1 70k protein that is both necessary and sufficient for ASF/SF2 binding is characterized, and a domain within this segment, which begins with Arg240 and ends with Asp270, was shown to bind specifically to the arginine/serine domain of ASF /SF2 using a yeast two-hybrid system and a far Western assay.
Characterization of a novel arginine/serine-rich splicing factor in Arabidopsis.
TLDR
The isolation of this new plant splicing factor family enlarges the essential group of RS domain splicing factors and suggests that these proteins play key roles in constitutive and alternative splicing in plants.
Serine-Arginine (SR) Protein-like Factors That Antagonize Authentic SR Proteins and Regulate Alternative Splicing*
TLDR
It is proposed that these two RNA-binding proteins are SR protein-like alternative splicing regulators that antagonize authentic SR proteins in the modulation of alternative 5′ splice site choice.
Functional properties of p54, a novel SR protein active in constitutive and alternative splicing
TLDR
It is reported that p54 is a new member of the SR family of splicing factors, as judged from its structural, antigenic, and functional characteristics, and it is suggested that this novel SR protein may participate in regulation of alternative splicing in a tissue- and substrate-dependent manner.
An early ancestor in the evolution of splicing: a Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing.
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References

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