Characterization and cloning of the E11 antigen, a marker expressed by rat osteoblasts and osteocytes.

@article{Wetterwald1996CharacterizationAC,
  title={Characterization and cloning of the E11 antigen, a marker expressed by rat osteoblasts and osteocytes.},
  author={Antoinette Wetterwald and W Hoffstetter and Marco Antonio G Cecchini and Beate Lanske and Claudia Wagner and Herbert Andre Fleisch and Mark Atkinson},
  journal={Bone},
  year={1996},
  volume={18 2},
  pages={125-32}
}
A new marker for cells of the osteoblastic lineage was identified by raising monoclonal antibodies against an immortalized rat osteoblastic cell line. Among the different antibodies one was selected which, on tissue sections, strongly reacts with osteoblasts, preosteocytes, and osteocytes. This antibody, designated E11, recognizes an antigen localized at the cell surface. The cDNA encoding the E11 antigen was cloned from a cDNA library prepared from ROS 17/2.8 cells, using a eukaryotic… CONTINUE READING
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