Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis.

@article{Bellamine1999CharacterizationAC,
  title={Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis.},
  author={Aouatef Bellamine and Akshay Mangla and William David Nes and Michael R. Waterman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 16},
  pages={8937-42}
}
Sterol 14alpha-demethylase encoded by CYP51 is a mixed-function oxidase involved in sterol synthesis in eukaryotic organisms. Completion of the Mycobacterium tuberculosis genome project revealed that a protein having homology to mammalian 14alpha-demethylases might be present in this bacterium. Using genomic DNA from mycobacterial strain H(37)Rv, we have established unambiguously that the CYP51-like gene encodes a bacterial sterol 14alpha-demethylase. Expression of the M. tuberculosis CYP51… CONTINUE READING