Characterization and activity of angiotensin-converting enzyme in Holstein semen.

@article{Costa2012CharacterizationAA,
  title={Characterization and activity of angiotensin-converting enzyme in Holstein semen.},
  author={Deiler Sampaio Costa and Jacob C Thundathil},
  journal={Animal reproduction science},
  year={2012},
  volume={133 1-2},
  pages={
          35-42
        }
}
The study was designed to perform immunodetection in spermatozoa and seminal plasma, immunolocalization in spermatozoa, and evaluation of the enzymatic activity of angiotensin-converting enzyme (ACE) in the semen of Holstein bulls. We used ejaculates from five bulls as part of a regular collection of semen. The monoclonal anti-ACE antibody recognized a single protein band with 100 kDa in detergent extract prepared from sperm and in seminal plasma. ACE enzymatic activity in sperm was 43.7, 21.3… CONTINUE READING

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