Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells

  title={Characterization and Quenching of Autofluorescence in Piglet Testis Tissue and Cells},
  author={Yanfei Yang and A. Honaramooz},
  journal={Anatomy Research International},
  • Yanfei Yang, A. Honaramooz
  • Published 2012
  • Biology, Medicine
  • Anatomy Research International
  • Significant intrinsic fluorescence in tissues and in disassociated cells can interfere with fluorescence identification of target cells. The objectives of the present study were (1) to examine an intrinsic fluorescence we observed in both the piglet testis tissue and cells and (2) to test an effective method to block the autofluorescence. We observed that a number of granules within the testis interstitial cells were inherently fluorescent, detectable using epifluorescence microscopy, confocal… CONTINUE READING
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