Characterization and Genetic Variation of Vibrio cholerae Isolated from Clinical and Environmental Sources in Thailand

@article{Siriphap2017CharacterizationAG,
  title={Characterization and Genetic Variation of Vibrio cholerae Isolated from Clinical and Environmental Sources in Thailand},
  author={Achiraya Siriphap and P. Leekitcharoenphon and Rolf Sommer Kaas and Chonchanok Theethakaew and Frank M. Aarestrup and Orasa Sutheinkul and Rene S. Hendriksen},
  journal={PLoS ONE},
  year={2017},
  volume={12}
}
Cholera is still an important public health problem in several countries, including Thailand. In this study, a collection of clinical and environmental V. cholerae serogroup O1, O139, and non-O1/non-O139 strains originating from Thailand (1983 to 2013) was characterized to determine phenotypic and genotypic traits and to investigate the genetic relatedness. Using a combination of conventional methods and whole genome sequencing (WGS), 78 V. cholerae strains were identified. WGS was used to… 

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References

SHOWING 1-10 OF 84 REFERENCES
Population Structure and Evolution of Non-O1/Non-O139 Vibrio cholerae by Multilocus Sequence Typing
TLDR
The MLST scheme developed in this study provides a framework to identify sporadic cholera isolates by genetic identity and was applied to 55 non-O1/non-O139 Vibrio cholerae isolates from clinical and environmental sources.
Drug response and genetic properties of Vibrio cholerae associated with endemic cholera in north-eastern Thailand, 2003-2011.
TLDR
Four clonal types, mostly of environmental origin, including subtypes, reflected genetic diversity, while distinct signatures were observed for clonally related, altered ET from Thailand, Vietnam and Bangladesh, confirmed by distinct subclustering patterns observed in the PFGE (NotI)-based dendrogram, suggesting that endemic cholera is caused by V. cholerae indigenous to Khon Kaen.
Population Structure and Evolution of NonO 1 / NonO 139 Vibrio cholerae by Multilocus Sequence Typing
TLDR
The MLST scheme developed in this study provides a framework to identify sporadic cholera isolates by genetic identity and Population Structure and Evolution of Non-O1/Non-O139 Vibrio cholerae by Multilocus Sequence Typing.
Vibrio cholerae O1 Hybrid El Tor Strains, Asia and Africa
TLDR
This work examined 41 clinical V. cholerae strains from Asia and Africa that were isolated from 1991 through 2004 and confirmed as serogroup O1 by O-antigen biosynthesis gene (rfbO1)-specific PCR, and found that all strains were confirmed as El Tor.
Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area
TLDR
Results of this study suggest that the environmental V. cholerae population in a cholera-endemic area is highly heterogeneous, and selection in the mammalian intestine can cause enrichment of environmental strains with virulence potential, which involves more virulence genes than currently appreciated.
Molecular analysis of non-O1/non-O139 Vibrio cholerae isolated from hospitalised patients in China
TLDR
The genetic diversity of non-O1/non-O139 V. cholerae isolates from hospitalised diarrhoeal patients in Zhejiang Province, China was determined and it was concluded that resistance to commonly used antibiotics limits treatment options.
Molecular Analysis of Vibrio choleraeO1, O139, non-O1, and non-O139 Strains: Clonal Relationships between Clinical and Environmental Isolates
TLDR
It was concluded that the non-cholera-toxin-producing strains of V. cholerae, whether of clinical or environmental origin, possess the ability to produce a new secretogenic toxin that is entirely different from the toxin produced by toxigenic V. Cholerae.
Isolation and characterization of Vibrio cholerae isolates from seafood in Hat Yai City, Songkhla, Thailand.
TLDR
This study isolated and characterized V. cholerae from seafood obtained from several markets in Hat Yai city, Southern Thailand, using the enterobacterial repetitive intergenic consensus-PCR method.
Vibrio cholerae O1 El Tor variant and emergence of Haitian ctxB variant in the strains isolated from South India
TLDR
The emergence of new variant form of V. cholerae O1 El Tor biotype with a novel mutation in ctxB in strains isolated from various outbreaks during 2010–2014 in Belgaum situated in north-west Karnataka, India is reported.
Molecular Typing of Vibrio cholerae O1 Isolates from Thailand by Pulsed-field Gel Electrophoresis
TLDR
The sustained appearance of the pathogenic V. cholerae O1 clone PF-II, the disappearance of clones PF-I and PF-III, and the emergence of new pathogenic clones during the two outbreaks of cholera are documented.
...
...