Characterization and Analysis of Posttranslational Modifications of the Human Large Cytoplasmic Ribosomal Subunit Proteins by Mass Spectrometry and Edman Sequencing

@article{Odintsova2003CharacterizationAA,
  title={Characterization and Analysis of Posttranslational Modifications of the Human Large Cytoplasmic Ribosomal Subunit Proteins by Mass Spectrometry and Edman Sequencing},
  author={Tatyana I. Odintsova and E C M{\"u}ller and Anton V. Ivanov and Tsezi A. Egorov and Ralf Bienert and Serguei Vladimirov and Susanne Kostka and Albrecht Otto and Brigitte Dr Wittmann-Liebold and Galina G. Karpova},
  journal={Journal of Protein Chemistry},
  year={2003},
  volume={22},
  pages={249-258}
}
The 60S ribosomal proteins were isolated from ribosomes of human placenta and separated by reversed phase HPLC. The fractions obtained were subjected to trypsin and Glu-C digestion and analyzed by mass fingerprinting (MALDI-TOF), MS/MS (ESI), and Edman sequencing. Forty-six large subunit proteins were found, 22 of which showed masses in accordance with the SwissProt database (June 2002) masses (proteins L6, L7, L9, L13, L15, L17, L18, L21, L22, L24, L26, L27, L30, L32, L34, L35, L36, L37, L37A… 

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