Characterization, performance, and applications of a yeast surface display-based biocatalyst

@inproceedings{Eby2015CharacterizationPA,
  title={Characterization, performance, and applications of a yeast surface display-based biocatalyst},
  author={Jonathan M Eby and Steven W. Peretti},
  year={2015}
}
This work demonstrates the efficacy and cost effectiveness of yeast surface display (YSD) as a method for producing and purifying enzyme catalysts. Lipase B from Candida antarctica (CalB) and lipase from Photobacterium lipolyticum sp. M37 (M37L) were individually displayed on the surface of yeasts via fusion with alpha-agglutinin. The enzyme is produced, purified, and immobilized in a single step. The population expressing the enzyme was quantified by flow cytometry. After lyophilization, the… CONTINUE READING