Characteristics of the spin-trapping reaction of a free radical derived from AAPH: further development of the ORAC-ESR assay.

@article{Nakajima2012CharacteristicsOT,
  title={Characteristics of the spin-trapping reaction of a free radical derived from AAPH: further development of the ORAC-ESR assay.},
  author={Akira Nakajima and Eriko Matsuda and Yasushi Masuda and Hisayo Sameshima and Tatsuya Ikenoue},
  journal={Analytical and bioanalytical chemistry},
  year={2012},
  volume={403 7},
  pages={1961-70}
}
The characteristics of the spin-trapping reaction in the oxygen radical absorbance capacity (ORAC)-electron spin resonance (ESR) assay were examined, focusing on the kind of spin traps. 2,2-Azobis(2-amidinopropane) dihydrochloride (AAPH) was used as a free radical initiator. The spin adducts of the AAPH-derived free radical were assigned as those of the alkoxyl radical, RO· (R=H(2)N(HN)C-C(CH(3))(2)). Among the spin traps tested, 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 5,5-dimethyl-4-phenyl-1… CONTINUE READING

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