Characteristics of sterol uptake in Saccharomyces cerevisiae.
@article{Lorenz1986CharacteristicsOS, title={Characteristics of sterol uptake in Saccharomyces cerevisiae.}, author={R. Lorenz and R. J. Rodriguez and T. Lewis and L. Parks}, journal={Journal of bacteriology}, year={1986}, volume={167 3}, pages={ 981-5 } }
A Saccharomyces cerevisiae sterol auxotroph, FY3 (alpha hem1 erg7 ura), was used to probe the characteristics of sterol uptake in S. cerevisiae. The steady-state cellular concentration of free sterol at the late exponential phase of growth could be adjusted within a 10-fold range by varying the concentration of exogenously supplied sterol. When cultured on 1 microgram of sterol ml-1, the cells contained a minimal cellular free-cholesterol concentration of 0.85 nmol/mg (dry weight) and were… CONTINUE READING
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