Characteristics of rat carcinoma in culture.

  title={Characteristics of rat carcinoma in culture.},
  author={Adel A. Yunis and Grace K. Arimura and Heather Haines and R J Ratzan and Michael A. Gross},
  journal={Cancer research},
  volume={35 2},
Rat chloroma cells have been propagated in permanent suspension cultures and are grown in Dulbecco's modified Eagle's medium with 10% fetal calf serum and 2.5% horse serum. Several lines have been established; the one of longest duration, Mia C51, has been maintained for over 18 months and has undergone over 100 transfers. Mia C51 cells have a doubling time of 12 hr and maintain many of the properties of the parent tumor, including the characteristic greenish color with high myeloperoxidase… CONTINUE READING
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