Characteristics of preimplantational development of porcine parthenogenetic diploids relative to the existence of amino acids in vitro.

@article{Thuan2002CharacteristicsOP,
  title={Characteristics of preimplantational development of porcine parthenogenetic diploids relative to the existence of amino acids in vitro.},
  author={Nguyen Van Thuan and Hiroshi Harayama and Masashi Miyake},
  journal={Biology of reproduction},
  year={2002},
  volume={67 6},
  pages={1688-98}
}
The present study was designed to investigate the effects of amino acids on the in vitro development of porcine parthenogenetic diploids that were produced by electrostimulation (El-St) and cytochalasin B treatment of in vitro-matured oocytes. The culture medium for development, based on Whitten medium, contained 0.5 mg/ml of hyaluronic acid (mWM), and a two-step culture system in which 290 mOsmol before the 4-cell stage (48 or 72 h after El-St) and, subsequently, 256 mOsmol up to the… CONTINUE READING

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