Characteristics of novel lignin peroxidases produced by white-rot fungus Phanerochaete sordida YK-624.

@article{Hirai2005CharacteristicsON,
  title={Characteristics of novel lignin peroxidases produced by white-rot fungus Phanerochaete sordida YK-624.},
  author={Hirofumi Hirai and Mutsumi Sugiura and Shingo Kawai and Tomoaki Nishida},
  journal={FEMS microbiology letters},
  year={2005},
  volume={246 1},
  pages={19-24}
}
We characterized a lignin peroxidase (YK-LiP2) isolated from shaking culture inoculated with the white-rot fungus Phanerochaete sordida YK-624. The YK-LiP2 enzyme was identified and purified to homogeneity by anion-exchange chromatography and gel permeation chromatography. The molecular weight of YK-LiP2 was approximately 45 kDa, and its absorption spectrum was almost the same as that of the LiP (Pc-LiP) from P. chrysosporium. Steady-state kinetics of veratryl alcohol (VA) oxidation by YK-LiP2… CONTINUE READING

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Moreover , YK - LiP2 and YK - LiP1 , which was previously isolated from static culture inoculated with P. sordida YK-624 , oxidized VA under a higher concentration of hydrogen peroxide ( > 2.5 mM ) although Pc - LiP could not oxidize VA in the presence of 2.5 mM hydrogen peroxide .
Moreover , YK - LiP2 and YK - LiP1 , which was previously isolated from static culture inoculated with P. sordida YK-624 , oxidized VA under a higher concentration of hydrogen peroxide ( > 2.5 mM ) although Pc - LiP could not oxidize VA in the presence of 2.5 mM hydrogen peroxide .
Moreover , YK - LiP2 and YK - LiP1 , which was previously isolated from static culture inoculated with P. sordida YK-624 , oxidized VA under a higher concentration of hydrogen peroxide ( > 2.5 mM ) although Pc - LiP could not oxidize VA in the presence of 2.5 mM hydrogen peroxide .
Moreover , YK - LiP2 and YK - LiP1 , which was previously isolated from static culture inoculated with P. sordida YK-624 , oxidized VA under a higher concentration of hydrogen peroxide ( > 2.5 mM ) although Pc - LiP could not oxidize VA in the presence of 2.5 mM hydrogen peroxide .
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