Characteristics of fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates

Abstract

Fed-batch cultures of recombinant Escherichia coli BL21 for producing human-like collagen were performed at different specific growth rates (0.1~0.25 h−1) before induction and at a constant value of 0.05 h−1 after induction by the method of pseudo-exponential feeding. Although the final biomass (around 69 g l−1) was almost the same in all fed-batch cultures… (More)
DOI: 10.1007/s10529-005-6720-8

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