Characteristics of fast mediated bioelectrocatalytic reaction near microelectrodes.

  title={Characteristics of fast mediated bioelectrocatalytic reaction near microelectrodes.},
  author={Yuki Kitazumi and Tatsuo Noda and Osamu Shirai and Masahiro Yamamoto and Kenji Kano},
  journal={Physical chemistry chemical physics : PCCP},
  volume={16 19},
The pseudo-steady-state current due to a mediated enzymatic reaction on a microelectrode is characterized on the basis of theoretical analysis and numerical simulation. The steady-state current is proportional to substrate concentration when the enzymatic reaction is considerably faster than substrate mass transport via nonlinear diffusion. Under such conditions, the reaction plane, where the mass flow of the substrate is converted to that of the mediator, exists near the electrode surface. The… 

Figures from this paper

Development Perspective of Bioelectrocatalysis-Based Biosensors

This review summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection and introduces prospective aspects of two new concepts of bios Sensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensor for absolute determination.

Enzymatic Bioelectrocatalysis

The basic feature and thermodynamics of redox proteins including redox enzymes (oxidoreductases) are introduced, and the basic concept of bioelectrocatalysis is introduced.



Diffusion-controlled detection of glucose with microelectrodes in mediated bioelectrocatalytic oxidation.

Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration.

A Theoretical Study on the Diffusion Current at the Stationary Electrodes of Circular and Narrow Band Types

The oxygen diffusion current on the platinum electrode was studied theoretically in both circular and band type electrodes. For the oxygen current of the circular electrode the following equation was

Fundamentals and Practices of Mediated Bioelectrocatalysis

Practical applications of the mediated bioelectrocatalysis system includes biosensors for enzyme substrates, amplified detection of mediators, bioreactors, biofuel cells, enzyme kinetic measurements, and protein redox potential measurements.