Characteristics of endogenous peptides eluted from the class I MHC molecule HLA-B7 determined by mass spectrometry and computer modeling.

@article{Huczko1993CharacteristicsOE,
  title={Characteristics of endogenous peptides eluted from the class I MHC molecule HLA-B7 determined by mass spectrometry and computer modeling.},
  author={Eric L. Huczko and Wanda M. Bodnar and David C. Benjamin and Kazuyasu Sakaguchi and Nongliao Zhu and Jeffrey Shabanowitz and Robert A. Henderson and Ettore Appella and Donald F. Hunt and Victor H Engelhard},
  journal={Journal of immunology},
  year={1993},
  volume={151 5},
  pages={2572-87}
}
Microcapillary HPLC electrospray ionization tandem mass spectrometry was used to sequence 15 peptides eluted from HLA-B7. Sequence alignment implicated four peptide positions in specific interactions with the class I molecule, and their importance was confirmed using synthetic peptides. Because no crystal structure for HLA-B7 was available, computer-assisted modeling was used to understand novel aspects of peptide binding specificity and to accurately predict the effect of defined changes in… CONTINUE READING

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