Characteristics of a two-site immunoradiometric assay for human skeletal alkaline phosphatase in serum.

  title={Characteristics of a two-site immunoradiometric assay for human skeletal alkaline phosphatase in serum.},
  author={Kalpana Panigrahi and Pierre Dominique Delmas and Franz Singer and William Ryan and Oscar K Reiss and Robin Fisher and Ph. D. Aubrey Miller and Itzhak Mizrahi and Claude Darte and Barry C. Kress},
  journal={Clinical chemistry},
  volume={40 5},
This two-site IRMA includes specific monoclonal antibodies for measuring skeletal alkaline phosphatase (B-ALP) in human serum. Assay calibration is based on mass units (micrograms per liter) and was established with purified B-ALP from a human osteosarcoma cell line, SAOS-2. Precision studies demonstrated intra- and interassay CVs of 3-5% and 5-7%, respectively. Relative reactivity studies showed that the assay has a sevenfold preference for detecting B-ALP compared with the liver isoenzyme in… CONTINUE READING


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