Characteristics of 2-oxoglutarate and glutamate transport in spinach chloroplasts

@article{Flgge1988CharacteristicsO2,
  title={Characteristics of 2-oxoglutarate and glutamate transport in spinach chloroplasts},
  author={I. U. Fl{\"u}gge and Kafai Woo and Hans Walter Heldt},
  journal={Planta},
  year={1988},
  volume={174},
  pages={534-541}
}
The transport of glutamate, 2-oxoglutarate and malate in intact spinach chloroplasts was determined using a double-silicone-layer centrifugation technique in which the silicone layers stayed separated at the end of centrifugation. Glutamate was found to be transported via the dicarboxylate but not the 2-oxoglutarate translocator. Hence the kinetic parameters (i.e.K m,K i andV max) determined in glutamate-preloaded chloroplasts represent the kinetic constants of the dicarboxylate translocator… CONTINUE READING
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References

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SHOWING 1-10 OF 19 REFERENCES

A mutant of Arab# dopsis deficient in chloroplast dicarboxylate transport is missing an envelope protein

  • S. C. Somerville, C. R. Somerville
  • Plant Sci. Lett
  • 1985
1 Excerpt

Kinetic characteristics of the chloroplast envelope glycolate transporter

  • K. T. Howitz, R. E. McCarty
  • Biochemistry
  • 1985
1 Excerpt

Methods of enzymatic analysis, 3rd edn

  • H. U. Bergmeyer
  • Verlag Chemie,
  • 1985
1 Excerpt

Kinetic studies on the transport of 2-oxoglutarate and L-malate into isolated pea chloroplasts

  • M. O. Proudlove, D. A. Thurman, J. Salisbury
  • New Phytol
  • 1984

Nichtw/iBrige Fraktionierung von Spinatblfittern. Eine Methode zur Bestimmung subzellul/irer Metabolitspiegel in vivo

  • R. Gerhardt
  • Ph.D. Dissertation. Universit/it G6ttingen,
  • 1984

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