Characteristics affecting expression and solubilization of yeast membrane proteins.

  title={Characteristics affecting expression and solubilization of yeast membrane proteins.},
  author={Michael A. White and Kathleen M Clark and Elizabeth J. Grayhack and Mark E Dumont},
  journal={Journal of molecular biology},
  volume={365 3},
Biochemical and structural analysis of membrane proteins often critically depends on the ability to overexpress and solubilize them. To identify properties of eukaryotic membrane proteins that may be predictive of successful overexpression, we analyzed expression levels of the genomic complement of over 1000 predicted membrane proteins in a recently completed Saccharomyces cerevisiae protein expression library. We detected statistically significant positive and negative correlations between… CONTINUE READING

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