Characteristic features of an alpha-galactosidase from mung beans.

  title={Characteristic features of an alpha-galactosidase from mung beans.},
  author={Prakash Mohan Dey},
  journal={European journal of biochemistry},
  volume={140 2},
Two molecular forms, I and II (high and low molecular mass) of alpha-galactosidase were demonstrated in dry mung beans and a multi-step procedure was developed for isolating the tetrameric enzyme I in good yield. Two affinity chromatographic techniques were employed and an overall 10 000-fold purification was achieved. The enzyme was able to catalyse the hydrolysis of alpha-galactosidic linkages as well as agglutinate rabbit erythrocytes (clot formation). The clot was temporary and dissolved on… CONTINUE READING

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