Characterising cytotoxic agent action as a function of the cell cycle using Fourier transform infrared microspectroscopy.

  title={Characterising cytotoxic agent action as a function of the cell cycle using Fourier transform infrared microspectroscopy.},
  author={M. Emilia Jim{\'e}nez-Hern{\'a}ndez and M D Brown and Caryn Hughes and Noel W. Clarke and Peter Gardner},
  journal={The Analyst},
  volume={140 13},
Fourier Transform Infrared (FTIR) micro-spectroscopy measurements were acquired to study infrared signatures of chemotherapeutic response as a function of the cell cycle. Renal carcinoma Caki-2 cells were exposed to IC50 doses of 5-fluorouracil and Paclitaxel for a period of 24 hours. The inherent cell cycle infrared signatures from untreated and drug-treated cells were successfully retrieved by the construction of a robust SVM able to discriminate the cell cycle phases of this cell line with… 

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