Characterisation of the wheat genome by renaturation kinetics

  title={Characterisation of the wheat genome by renaturation kinetics},
  author={Derek B. Smith and Richard B. Flavell},
Reassociation kinetics of sheared hexaploid wheat DNA in 0.18 M Na+ at 60 °C show the presence of three major classes of nucleotide sequences; (1) a very rapidly reannealing fracton that comprises about 4 to 10% of the genome and reanneals virtually instantly. This fraction contains sequences which appear to be randomly distributed through at least 40% of the genome; they may be palindromic sequences; (2) a heterogeneous intermediate reannealing fraction comprising about 70–80% of the genome… CONTINUE READING