Characterisation of the temporal sequence of osteoblast gene expression during estrogen-induced osteogenesis in female mice.

@article{Plant2001CharacterisationOT,
  title={Characterisation of the temporal sequence of osteoblast gene expression during estrogen-induced osteogenesis in female mice.},
  author={Andrea Plant and Jonathan H. Tobias},
  journal={Journal of cellular biochemistry},
  year={2001},
  volume={82 4},
  pages={683-91}
}
Osteoblast differentiation under in vitro conditions is associated with increased expression of non-collagenous bone proteins including osteocalcin, osteopontin, and osteonectin, the exact function of which remain poorly understood. To determine whether these proteins play an important role in the formation of mineralised bone matrix by osteoblasts in vivo, we analysed the time-course of their expression during estrogen-induced osteogenesis in female mice, and compared this with the formation… CONTINUE READING

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In parallel experiments , the time course of cancellous bone formation was determined by measuring bone mineral density ( BMD ) of the distal femur .
In parallel experiments , the time course of cancellous bone formation was determined by measuring bone mineral density ( BMD ) of the distal femur .
To determine whether these proteins play an important role in the formation of mineralised bone matrix by osteoblasts in vivo , we analysed the time - course of their expression during estrogen - induced osteogenesis in female mice , and compared this with the formation of new cancellous bone .
To determine whether these proteins play an important role in the formation of mineralised bone matrix by osteoblasts in vivo , we analysed the time - course of their expression during estrogen - induced osteogenesis in female mice , and compared this with the formation of new cancellous bone .
OsteoblastsAnatomic structure is physical part ofBone Tissue
Osteoblast differentiation under in vitro conditions is associated with increased expression of non - collagenous bone proteins including osteocalcin , osteopontin , and osteonectin , the exact function of which remain poorly understood .
Our results suggest that osteocalcin , osteopontin , and osteonectin are not involved in the initial phase of the osteogenic response to estrogen , suggesting that these non - collagenous bone proteins do not play a direct role in the formation of mineralised bone matrix by osteoblasts in vivo .
In contrast , osteocalcin , osteopontin , and osteonectin expression showed no change during this initial period , although modest increases were observed at later times ( i.e. , Days 20 and 24 ) .
In contrast , osteocalcin , osteopontin , and osteonectin expression showed no change during this initial period , although modest increases were observed at later times ( i.e. , Days 20 and 24 ) .
Our results suggest that osteocalcin , osteopontin , and osteonectin are not involved in the initial phase of the osteogenic response to estrogen , suggesting that these non - collagenous bone proteins do not play a direct role in the formation of mineralised bone matrix by osteoblasts in vivo .
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