Characterisation of the nucleotide and DNA coeffector binding sites of the herpes simplex virus type 1 (HSV-1) encoded helicase-primase complex and UL9 origin binding protein.


Nucleotide and DNA coeffector substrate binding site characterizations were performed on two HSV-1 DNA helicases fulfilling different roles in DNA replication. Single ATP-binding sites were identified for helicase-primase and UL9 protein (Km(ATP) 0.62 mM and 0.54 mM, respectively). Analysis of structural requirements for DNA-dependent NTP hydrolysis… (More)


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