Characterisation of secondary metabolites associated with neutrophil apoptosis.

  title={Characterisation of secondary metabolites associated with neutrophil apoptosis.},
  author={A V Nunn and Michele L. Barnard and Kishore Kumar Bhakoo and John Murray and E J Chilvers and Jimmy D. Bell},
  journal={FEBS letters},
  volume={392 3},
We studied changes in secondary metabolites in human neutrophils undergoing constitutive or tumour necrosis factor (TNFalpha) stimulated apoptosis by a combination of high-performance liquid chromatography (HPLC) and NMR spectroscopy. Our results show that in contrast to freshly isolated neutrophils, neutrophil cells aged for 20 h in vitro had marked differences in the levels of a number of endogenous metabolites including lactate, amino acids and phosphocholine (PCho). There was no change in… CONTINUE READING