Characterisation of rat hepatocyte monolayers for investigation of the metabolism of bile salts.

@article{Ford1985CharacterisationOR,
  title={Characterisation of rat hepatocyte monolayers for investigation of the metabolism of bile salts.},
  author={Renee Ford and Kathleen M. Botham and Keith E. Suckling and G. S. Boyd},
  journal={Biochimica et biophysica acta},
  year={1985},
  volume={836 2},
  pages={185-91}
}
Rat hepatocyte monolayers were maintained for periods up to 24 h during which time their viability was greater than 85%. Using specific radioimmunoassays, the hepatocyte monolayers were shown to synthesise conjugated cholic, chenodeoxycholic and beta-muricholic acids. Feeding the bile salt sequestrant, cholestyramine, to donor animals increased synthesis of the major bile salt conjugates by the cells. Incubation of hepatocyte monolayers with bovine serum albumin decreased total synthesis of the… CONTINUE READING