Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.
@article{Sokolova2011CharacterisationOE, title={Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.}, author={Viktoriya Sokolova and Anna-Kristin Ludwig and Sandra Hornung and Olga Rotan and Peter A. Horn and Matthias Epple and Bernd Giebel}, journal={Colloids and surfaces. B, Biointerfaces}, year={2011}, volume={87 1}, pages={ 146-50 } }
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References
SHOWING 1-10 OF 18 REFERENCES
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
- BiologyCurrent protocols in cell biology
- 2006
This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosomes preparations.
Characterization of exosomeālike vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense
- BiologyFASEB journal : official publication of the Federation of American Societies for Experimental Biology
- 2009
It is demonstrated for the first time that exosomeālike vesicles with antiviral properties are present in human tracheobronchial epithelial (HTBE) cell culture secretions and that these structures may be involved in diverse physiological processes in airway biology, including innate mucosal defense.
Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury.
- BiologyStem cell research
- 2010
The potential of exosomes in immunotherapy
- Biology, ChemistryExpert opinion on biological therapy
- 2005
This review will describe the biogenesis, the protein and lipid compositions, and the biological functions of exosomes, and present their immunostimulatory functions in mice and humans, and will discuss their possible use as cancer vaccines.
Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy.
- Biology, Materials ScienceACS nano
- 2010
The substructure of single human saliva exosomes is shown, using a new ultrasensitive low force atomic force microscopy (AFM) exhibiting substructural organization unresolvable in electron microscopy.
Exosomes/microvesicles as a mechanism of cell-to-cell communication.
- BiologyKidney international
- 2010
The transfer of gene products from injured cells may explain stem cell functional and phenotypic changes without the need of transdifferentiation into tissue cells, and the evidence supporting a bidirectional exchange of genetic information between stem and injured cells is discussed.
Possibilities and limitations of different analytical methods for the size determination of a bimodal dispersion of metallic nanoparticles
- Materials Science
- 2011
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
- BiologyNature Cell Biology
- 2007
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called āexosomal shuttle RNAā (esRNA).
Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: demonstration of a pathway for receptor shedding.
- Biology, MedicineEuropean journal of cell biology
- 1984
The data suggest that Tf receptors are shed from developing reticulocytes by incorporation into the limiting membrane of inclusion vesicle discharge, followed by discharge of these vesicles by MVE exocytosis, which matches Tf receptor loss rates closely enough to suggest that this is the primary path of receptor loss during Reticulocyte development.
Tumor-Derived Microvesicles Promote Regulatory T Cell Expansion and Induce Apoptosis in Tumor-Reactive Activated CD8+ T Lymphocytes1
- Biology, MedicineThe Journal of Immunology
- 2009
The data suggest that tumor-derived MV induce immune suppression by promoting T regulatory cell expansion and the demise of antitumor CD8+ effector T cells, thus contributing to tumor escape.