Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.

  title={Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.},
  author={Viktoriya Sokolova and Anna-Kristin Ludwig and Sandra Hornung and Olga Rotan and Peter A. Horn and Matthias Epple and Bernd Giebel},
  journal={Colloids and surfaces. B, Biointerfaces},
  volume={87 1},

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy.

Transmission electron microscopy is suggested for the imaging of exosomes including negative staining, whole mount immuno-staining, block preparation, thin section, and Immuno-gold labelling, to observe detailed structure.

Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.

This chapter describes a method for identifying the size and concentration of a subpopulation of vesicles in biological samples, using nanoparticle tracking analysis, enabled by specific marker antibodies, coupled to fluorescent quantum dots.

Elucidating diversity of exosomes: biophysical and molecular characterization methods.

This review summarizes, first, biophysical techniques based on spectroscopy and other principles, and second, antibody-based molecular techniques including flow cytometry, transmission electron microscopy and third, nanotechnology-dependent exosome characterization methodologies.

Characterization of extracellular vesicles and artificial nanoparticles with four orthogonal single-particle analysis platforms

Drawing conclusions about existing single-particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies are drawn.

Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms

Drawing conclusions about existing single‐particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies are drawn.

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation, immunoaffinity, and microfluidic technologies for the isolation of exosomes.

Electron Microscopy-Based Comparison and Investigation of the Morphology of Exosomes Derived from Hepatocellular Carcinoma Cells Isolated at Different Centrifugal Speeds

The results demonstrate that exosomes possess a nearly spherical shape and bilipid membranous vesicle with a concave cavity structure containing electron-dense and coated vesicles, suggesting the possible existence of subpopulations of exosome with specific functions.

Size-dependent cellular uptake of exosomes.

Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation.

The emphasis was focused on a method development to size-separate, characterize, and quantify small amounts of exosomes by asymmetrical-flow field-flow fractionation (AF4) technique coupled to a multidetection system (UV and MALS).

Exosomes derived from lung cancer cells: Isolation, characterization, and stability studies.




Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosomes preparations.

Characterization of exosome‐like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense

It is demonstrated for the first time that exosome‐like vesicles with antiviral properties are present in human tracheobronchial epithelial (HTBE) cell culture secretions and that these structures may be involved in diverse physiological processes in airway biology, including innate mucosal defense.

The potential of exosomes in immunotherapy

This review will describe the biogenesis, the protein and lipid compositions, and the biological functions of exosomes, and present their immunostimulatory functions in mice and humans, and will discuss their possible use as cancer vaccines.

Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy.

The substructure of single human saliva exosomes is shown, using a new ultrasensitive low force atomic force microscopy (AFM) exhibiting substructural organization unresolvable in electron microscopy.

Exosomes/microvesicles as a mechanism of cell-to-cell communication.

The transfer of gene products from injured cells may explain stem cell functional and phenotypic changes without the need of transdifferentiation into tissue cells, and the evidence supporting a bidirectional exchange of genetic information between stem and injured cells is discussed.

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells

It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).

Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: demonstration of a pathway for receptor shedding.

The data suggest that Tf receptors are shed from developing reticulocytes by incorporation into the limiting membrane of inclusion vesicle discharge, followed by discharge of these vesicles by MVE exocytosis, which matches Tf receptor loss rates closely enough to suggest that this is the primary path of receptor loss during Reticulocyte development.

Tumor-Derived Microvesicles Promote Regulatory T Cell Expansion and Induce Apoptosis in Tumor-Reactive Activated CD8+ T Lymphocytes1

The data suggest that tumor-derived MV induce immune suppression by promoting T regulatory cell expansion and the demise of antitumor CD8+ effector T cells, thus contributing to tumor escape.