Characterisation of drug resistance gene cassettes associated with class 1 integrons in clinical isolates of Escherichia coli from Taiwan, ROC.

@article{Chang2000CharacterisationOD,
  title={Characterisation of drug resistance gene cassettes associated with class 1 integrons in clinical isolates of Escherichia coli from Taiwan, ROC.},
  author={Cheng-Yen Chang and Linda Li Chang and Yoon Hwan Chang and Travis M. Lee and S. F. Chang},
  journal={Journal of medical microbiology},
  year={2000},
  volume={49 12},
  pages={1097-102}
}
The presence of class 1 integrons in clinical isolates of Escherichia coli was detected by PCR. Of 104 E. coli isolates from Kaohsiung, 54 (52%) carried class 1 integrons, with inserted DNA regions of 1-3 kb. These integrons were located on plasmids, as demonstrated by Southern hybridisation. DNA sequencing was used to identify the genetic content of the integron-variable regions. Different class 1 integrons contained various numbers, kinds and combinations of gene cassettes within their… CONTINUE READING
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