Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence

  title={Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence},
  author={David G. Swan and Ana M. Rodr{\'i}guez and Carmen Vilches and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Molecular and General Genetics MGG},
A gene (ORFB) from Streptomyces antibioticus (an oleandomycin producer) encoding a large, multifunctional polyketide synthase (PKS) was cloned and sequenced. Its product shows an internal duplication and a close similarity to the third subunit of the PKS involved in erythromycin biosynthesis by Saccharopolyspora erythraea, showing the equivalent nine active site domains in the same order along the polypeptide. An unusual feature of this ORF is the GC content of most of the sequence, which is… Expand
The candicidin gene cluster from Streptomyces griseus IMRU 3570.
Of particular interest is the presence of the CanP1 loading domain (the first described as responsible for the activation of an aromatic starter unit) and the polypeptide CanP3 (carrying modules for the formation of five out of seven conjugated double bonds). Expand
The candicidin gene cluster from Streptomyces griseus
Departamento de Ecologı!a, Gene! tica y Microbiologı!a, A; rea de Microbiologı!a, Facultad de Biologı!a, Universidad de Leo! n, 24071 Leo! n, Spain A 205 kb DNA region from Streptomyces griseus IMRUExpand
Approaches to stabilization of inter-domain recombination in polyketide synthase gene expression plasmids
Two different solutions for circumventing intra-plasmid recombination within the megalomicin PKS genes in Streptomyces coelicolor are presented, permitting reproducible scale-up to at least 5-l stirred-tank fermentation and a comparison of diketide precursor incorporation efficiencies between the erythromycin and megalomics PKSs. Expand
Organization of the biosynthetic gene cluster for the polyketide macrolide mycinamicin in Micromonospora griseorubida.
The nucleotide sequence of the mycinamicin biosynthetic gene cluster, in which there were 22 open reading frames (ORFs), was completely determined and it was suggested that the KS-AT original region was horizontally transferred into the PKS genes on the chromosomal DNA of several actinomycetes strains. Expand
Genetic Organization of the Biosynthetic Gene Cluster for the Antitumor Angucycline Oviedomycin in Streptomyces antibioticus ATCC 11891
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Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055: cluster analysis and assignment of functions.
Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption ofborA3. Expand
Biosynthesis, Regulation, and Genetics of Macrolide Production
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Biosynthetic engineering of polyketide synthases
This review summarises some of the published patents and patent applications in this relatively new field of polyketides, focusing both on the core technological issues governing success in this area and on specific examples of patents describing the use of these technologies to produce molecules of therapeutic significance. Expand
Modular Polyketide Synthases
Polyketide synthase and the non-ribosomal peptide synthetase; a combinatorial biosynthesis; a hybrid system for novel polyketide synthesis. Expand


6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme.
Analysis of the sequence supports the view that the erythromycin-producing polyketide synthase contains three multienzyme polypeptides, each of which accomplishes two successive cycles ofpolyketide chain extension. Expand
An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea
A novel organization of the erythromycin-producing polyketide synthase is revealed, which provides further insight into the mechanism of chain assembly. Expand
Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes
It is shown that cloned DNA coding for one synthase might be used as a hybridization probe for the isolation of others and that this is indeed possible and valuable in giving access to groups of linked biosynthetic genes. Expand
Streptomyces antibioticus contains at least three oleandomycin‐resistance determinants, one of which shows similarity with proteins of the ABC‐transporter superfamily
The results suggest that resistance to oleandomycin conferred by oleC (orf4) is probably due to an efflux transport system of the ABC‐transporter superfamily. Expand
Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2).
It is postulated that bldA specifies a tRNA that would recognize the codon UUA (for leucine), which is very rare in Streptomyces genes and generally contain greater than 70 mole% (G + C], suggesting a possible role for bld a in translational control of development. Expand
TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative, Streptomyces mutants.
It is shown that phenotypic expression of three disparate genes (carB, lacZ, and ampC) containing TTA codons depends strongly on bldA, which specifies a tRNA(Leu)-like gene product which would recognize the UUA codon. Expand
Modular organization of genes required for complex polyketide biosynthesis.
In Saccharopolyspora erythraea, the genes that govern synthesis of the polyketide portion of the macrolide antibiotic erythromycin are organized in six repeated units that encode fatty acid synthaseExpand
Codon usage in the G+C-rich Streptomyces genome.
It appears that codon usage in Streptomyces is determined predominantly by mutation bias, with weak translational selection operating only in highly expressed genes. Expand
Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.
The structure of the biotechnology of POL YKETIDE SYNTHESIS is studied to understand the role of polyketides in plant growth and their role in the environment. Expand