Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence

@article{Swan2004CharacterisationOA,
  title={Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence},
  author={David G. Swan and Ana M. Rodr{\'i}guez and Carmen Vilches and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={242},
  pages={358-362}
}
A gene (ORFB) from Streptomyces antibioticus (an oleandomycin producer) encoding a large, multifunctional polyketide synthase (PKS) was cloned and sequenced. Its product shows an internal duplication and a close similarity to the third subunit of the PKS involved in erythromycin biosynthesis by Saccharopolyspora erythraea, showing the equivalent nine active site domains in the same order along the polypeptide. An unusual feature of this ORF is the GC content of most of the sequence, which is… Expand
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